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Chi-Hsien Liu



Chi-Hsien Liu, Professor 

Education: PhD. of Chemical Engineering; National Tsing

Hua   University,Taiwan

Interests:  Stem cell technology, Nanotechnology,

Drug delivery, Biochemical engineering

Telephone: 886-3-2118800 Ext: 3146

E-mail: chl@mail.cgu.edu.tw   

Laboratory: Cell Engineering Lab



09/1998-06/2001      Ph.D. in Chemical Engineering; National Tsing Hua University, Taiwan

09/1990-06/1992      M.S. in Chemical Engineering; Tatung University , Taiwan

09/1986-06/1990       B.S. in Bio Engineering; Tatung University , Taiwan



08/2018-present  Professor, Department Chemical and Materials Engineering ; Chang Gung University, Taiwan

08/2013-07/2018  Professor, Institute of Biochemical and Biomedical Engineering; Chang Gung University, Taiwan

08/2007-07/2013   Associate Professor, Institute of Biochemical and Biomedical  Engineering; Chang Gung University, Taiwan

08/2003-07/2007   Assistant Professor, Institute of Biochemical and Biomedical Engineering; Chang Gung University, Taiwan

08/2002-07/2003   Scientist Fellow, Food Industry Research Development Institute (FIRDI)

10/2001-07/2002   R&D manager, Widex Biotech. Co.

04/2001-09/2001   Project manager, Analytica Biotech. Co.

08/1992-08/1998  Associate Scientist Fellow, FIRDI



Drug delivery

Stem cell technology

Biochemical engineering




Member, Biochemical Engineering Society of Taiwan (BEST)

Member, Biomedical Engineering Society of ROC

Overseas Member, Society for Biotechnology, Japan



Journal Reviewer, Biochemical Engineering Journal

Journal Reviewer, Process Biochemistry

Journal Reviewer, Cytotherapy

Journal Reviewer, International Journal of Pharmaceutics

Journal Reviewer, Journal of the Chinese Institute of Chemical Engineers

Conference Reviewer, Annual Symposium of the Biochemical Engineering Society of Taiwan



Special contribution Award for FIRDI; (1998)

Program Execution Award from Ministry of Economic Affairs (1998)



1. Chen, G. C., Liu, C. H.*, Wu, W. C. Electrochemical immunosensor for serum parathyroid hormone using voltammetric techniques and a portable simulator. Analytica Chimica Acta, Volume 1143, 25, 84-92 (2021), doi: 10.1016/j.aca.2020.11.045, IF 5.977, CHEMISTRY, ANALYTICAL 10/86

2. Malla, P., Chen, G. C., Liao, H. P., Liu, C. H.*, Wu, W. C. Electrochemical immunoassay for serum parathyroid hormone using screen-printed carbon electrode and magnetic beads. Journal of Electroanalytical Chemistry, 895, 115463 (2021), doi: 10.1016/j.jelechem.2021.115463, IF3.807, CHEMISTRY, ANALYTICAL 17/86

3. Malla, P., Chen, G. C., Liao, H. P., Liu, C. H.*, Wu, W. C. Label-free parathyroid hormone immunosensor using nanocomposite modified screen-printed carbon electrode. Journal of Electroanalytical Chemistry, 880, 114917 (2021), doi: 10.1016/j.jelechem.2020.114917, IF3.807, CHEMISTRY, ANALYTICAL 17/86

4. Kumari, M., Liu, C. H.*, Wu, W. C., Wang, C. C. Gene delivery using layer-by-layer functionalized multi-walled carbon nanotubes: Design, characterization, cell line evaluation, Journal of Materials Science, 56, 7022–7033(2021), doi: 10.1007/s10853-020-05648-6, IF3.553, MATERIALS SCIENCE, MULTIDISCIPLINARY 108/314

5. Fan, Y. Y., Liu, C. H, Wu, A. L., Chen, H. C., Hsueh, Y. J.,Chen, K. J.,Lai, C. C.,Huang, C. Y., Wu, W. C.* MicroRNA-126 inhibits pathological retinal neovascularization via suppressing vascular endothelial growth factor expression in a rat model of retinopathy of prematurity, European Journal of Pharmacology, 900, 174035 (2021), doi: 10.1016/j.ejphar.2021.174035, IF 3.263

6. Liu, C. H.*, Lee, G. W., Wu, W. C., Wang, C. C. Encapsulating curcumin in ethylene diamine-β-cyclodextrin nanoparticle improves topical cornea delivery. Colloids and Surfaces B: Biointerfaces 186, 110726, doi: 10.1016/j.colsurfb.2019.110726 (2020). IF 4.389, BIOPHYSICS 12/71.

7. Kumari, M., Liu, C. H.*, Wu, W. C. Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility. International Journal of Biological Macromolecules, 142, 492-502, doi: 10.1016/j.ijbiomac.2019.09.121 (2020). IF 5.162, POLYMER SCIENCE 9/89

8. Sahoo, S. L., Liu, C. H.* Wu, W. C., Wang, C. C. Biocompatible quantum dot-antibody conjugate for cell imaging, targeting and fluorometric immunoassay: crosslinking, characterization and applications. RSC Advances 9, 32791-32803, doi: 10.1039/C9RA07352C (2019). 

9. Liu, C. H.*, Liu, Y.-X., Kumari, M., Wu, W.-C. Multivariate analysis of metabolic parameters and optimization of antibody production using high cell density hybridoma in hollow fiber bioreactors. Biotechnology Letters 41, 963-977, doi: 10.1007/s10529-019-02712-3 (2019). 

10. Liu, C. H.*, Liu, Y. X., Wu, W. C. Facile development of medium optimization for antibody production: Implementation in spinner flask and hollow fiber reactor, Cytotechnology, 2018, 70, 1631-1642 Biotechnology & Applied Microbiology, 119/160 (74 %) IF 1.461

11. Kumari, M., Liu, C. H.*, Wu, W. C. Protein moiety in oligochitosan modified vector regulates internalization mechanism and gene delivery: Polyplex characterization, intracellular trafficking and transfection. Carbohydrate Polymers, 2018, 202, 143-156 Chemistry, Applied, IF 7.182 CHEMISTRY, ORGANIC 2/57 

12. Rout, B., Liu, C. H.*, Wu, W. C. Increased anti-biofilm efficacy of toluidine blue on Staphylococcus species after nano-encapsulation. Photodiagnosis and Photodynamic Therapy, 2018, 21(3) 190–200, IF 2.895, Oncology 127/222 (Available online 13 December 2017)

13. Kumari, M., Liu, C. H.*, Wu, W. C. Efficient gene delivery by oligochitosan conjugated serum albumin: Facile synthesis, polyplex stability, and transfection, Carbohydrate Polymers, 2018, 183(3) 37-49, Chemistry, Applied, 2/71 (3%) (Available online 04 November 2017), IF 5.158,

14. Rout, B., Liu, C. H.*, Wu, W. C. Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function, Scientific Reports, 2017, 7, Article number: 7892, doi: 10.1038/s41598-017-07444-w, Published online: 11 August 2017, IF 4.259, Multidisciplinary Sciences 10/64 (16%)

15. Sahoo, S. L, Liu, C. H.*, Wu, W.C., 2017, Lymphoma cell isolation using multifunctional magnetic nanoparticles: antibody conjugation and characterization. RCS Advances, 7, 22468–22478.online 13 Apr 2017 IF 3.108, Chemistry, Multidisciplinary 59/166 (36%)

16. Liu, C. H.*, Lai, H.Y., Wu, W.C., 2017, Facile Synthesis of magnetic iron oxide nanoparticles for nattokinase isolation, Food and Bioproducts Processing, 102,260-267, IF 1.97, Food Science & Technology, 45/128 (35%),  Engineering, Chemical 56/135 (41%) online 1 March 2017

17. Liu, C. H.*, Tsao, M.H., Sahoo, S. L, Wu, W. C. 2017, Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining, RSC Advances, 7, 5937-5947 (published online 17 Jan 2017) IF 3.108, Chemistry, Multidisciplinary 59/166 (36%)

18. Hwang Y. S., Liu, C. H, Huang Y. C., Chen C. S., Chen T. L., Wang N. K., Chen Y. P., Chen K. J., Lai C. C., Wu W. C.*, 2017, Systemic effects after intravitreal injection of bevacizumab in new born rabbit eyes. Cutaneous and Ocular Toxicology, IF1.213, Ophthalmology 47/59 (80%)



Liu, C. H., Hwang, S. M., Serum-free cell culture system, In: First Mr. Hsieh Memorial Award  Proceeding. p201-228, 2004


1. Optimizing culture medium for CD34+ hematopoietic cell expansion. 2014, USP 8,762,074 B2

2. Purification methods of blood plasminogen, 2014, ROC Patent I460188

3. Microorganisms and process for fermented black tea beverage, 2000, Japan Patent 3084348 ; ROC Patent 385335